Western blot analysis of extracts of various cell lines, using CAST antibody (A7634) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Western blot analysis of extracts from normal (control) and CAST knockout (KO) HeLa cells, using CAST antibody (A7634) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] CAST Rabbit pAb
Catalog No:AB-A7634
Manufacturer:ABclonal
Synonyms:CAST;BS-17;PLACK
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] CAST Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1). |
Gene ID: | 831 |
Swiss prot: | P20810 |
Molecular weight: | 63kDa/71- 84kDa |
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