Western blot analysis of extracts of various cell lines, using CHFR antibody (A10447) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
CHFR Rabbit pAb
Catalog No:AB-A10447
Manufacturer:ABclonal
Synonyms:CHFR;RNF116;RNF196
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CHFR Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human CHFR (NP_001154818.1). |
Gene ID: | 55743 |
Swiss prot: | Q96EP1 |
Molecular weight: | 63kDa/69kDa/72kDa/73kDa |
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