Western blot analysis of extracts of various cell lines, using ATP6V0A4 antibody (A7591) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
ATP6V0A4 Rabbit pAb
Catalog No:AB-A7591
Manufacturer:ABclonal
Synonyms:ATP6V0A4;A4;ATP6N1B;ATP6N2;RDRTA2;RTA1C;RTADR;STV1;VPH1;VPP2
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ATP6V0A4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c’, c”, and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP6V0A4 (NP_065683.2). |
Gene ID: | 50617 |
Swiss prot: | Q9HBG4 |
Molecular weight: | 96kDa |
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