Western blot analysis of extracts of MCF-7 cells, using Cytokeratin 6 (KRT6) antibody (A7560) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Cytokeratin 6 (KRT6) Rabbit pAb
Catalog No:AB-A7560
Manufacturer:ABclonal
Synonyms:KRT6A;CK-6C;CK-6E;CK6A;CK6C;CK6D;K6A;K6C;K6D;KRT6C;KRT6D;PC3
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Cytokeratin 6 (KRT6) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 305-564 of human Cytokeratin 6 (KRT6) (NP_005545.1). |
Gene ID: | 3853 |
Swiss prot: | P02538 |
Molecular weight: | 60kDa |
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