Western blot analysis of extracts of THP-1 cells, using CD34 antibody (A7429) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Western blot analysis of extracts from normal (control) and CD34 Rabbit pAb knockout (KO) 293T cells, using CD34 Rabbit pAb antibody (A7429) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
[KO Validated] CD34 Rabbit pAb
Catalog No:AB-A7429
Manufacturer:ABclonal
Synonyms:CD34;CD34 molecule;GIG3;MORT1
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] CD34 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-320 of human CD34 (NP_001020280). |
Gene ID: | 947 |
Swiss prot: | P28906 |
Molecular weight: | 35kDa/40kDa |
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