Western blot analysis of extracts of various cell lines, using PRKAG1 antibody (A7300) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
PRKAG1 Rabbit pAb
Catalog No:AB-A7300
Manufacturer:ABclonal
Synonyms:PRKAG1;AMPKG
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PRKAG1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human PRKAG1 (NP_002724.1). |
Gene ID: | 5571 |
Swiss prot: | P54619 |
Molecular weight: | 34kDa/37kDa/38kDa |
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