Western blot analysis of extracts of various cell lines, using MCP-1 antibody (A7277) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
MCP-1 Rabbit pAb
Catalog No:AB-A7277
Manufacturer:ABclonal
Synonyms:CCL2;GDCF-2;HC11;HSMCR30;MCAF;MCP-1;MCP1;SCYA2;SMC-CF
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MCP-1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human MCP-1 (NP_002973.1). |
Gene ID: | 6347 |
Swiss prot: | P13500 |
Molecular weight: | 11kDa |
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