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Western blot analysis of extracts of HeLa cells, using Asymmetric DiMethyl-Histone H4-R3 antibody (A7261) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 300s.
Asymmetric DiMethyl-Histone H4-R3 Rabbit pAb
Catalog No：AB-A7261
Manufacturer：ABclonal
Synonyms：FO108;H4;H4/n;H4F2;H4FN;HIST2H4;Histone H4;HIST1H4A;HIST2H4A
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat,Other (Wide Range)
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Asymmetric DiMethyl-Histone H4-R3 Rabbit pAb |
| Category: | Methylated Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. |
| Immunogen: | A synthetic peptide of human HIST2H4A |
| Gene ID: | 8370 |
| Swiss prot: | P62805 |
| Molecular weight: | 11kDa |
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