Western blot analysis of extracts of various cell lines, using UCP1 antibody (A7236) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
UCP1 Rabbit pAb
Catalog No:AB-A7236
Manufacturer:ABclonal
Synonyms:UCP1;SLC25A7;UCP
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €255,00
Product Name: | UCP1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human UCP1 (NP_068605.1). |
Gene ID: | 7350 |
Swiss prot: | P25874 |
Molecular weight: | 33kDa |
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