Western blot analysis of extracts of various cell lines, using REST/NRSF antibody (A7161) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
REST/NRSF Rabbit pAb
Catalog No:AB-A7161
Manufacturer:ABclonal
Synonyms:REST;NRSF;WT6;XBR
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | REST/NRSF Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 760-1060 of human REST/NRSF (NP_001180437.1). |
Gene ID: | 5978 |
Swiss prot: | Q13127 |
Molecular weight: | 35kDa/36kDa/119kDa/121kDa |
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