Western blot analysis of extracts of various cell lines, using VPS35 antibody (A7117) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
VPS35 Rabbit pAb
Catalog No:AB-A7117
Manufacturer:ABclonal
Synonyms:VPS35;MEM3;PARK17
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | VPS35 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human VPS35 (NP_060676.2). |
Gene ID: | 55737 |
Swiss prot: | Q96QK1 |
Molecular weight: | 91kDa |
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