Western blot analysis of extracts of various cell lines, using VPS4A antibody (A7096) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
VPS4A Rabbit pAb
Catalog No:AB-A7096
Manufacturer:ABclonal
Synonyms:VPS4A;SKD1;SKD1A;SKD2;VPS4;VPS4-1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | VPS4A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human VPS4A (NP_037377.1). |
Gene ID: | 27183 |
Swiss prot: | Q9UN37 |
Molecular weight: | 48kDa |
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