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Western blot analysis of extracts of various cell lines, using USP8 antibody (A7031) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
USP8 Rabbit pAb
Catalog No：AB-A7031
Manufacturer：ABclonal
Synonyms：USP8;HumORF8;SPG59;UBPY
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | USP8 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human USP8 (NP_005145.3). |
| Gene ID: | 9101 |
| Swiss prot: | P40818 |
| Molecular weight: | 115kDa/127kDa |
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