Western blot analysis of extracts of various cell lines, using PSMD8 antibody (A6955) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PSMD8 Rabbit pAb
Catalog No:AB-A6955
Manufacturer:ABclonal
Synonyms:PSMD8;HEL-S-91n;HIP6;HYPF;Nin1p;Rpn12;S14;p31
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PSMD8 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 101-350 of human PSMD8 (NP_002803.2). |
Gene ID: | 5714 |
Swiss prot: | P48556 |
Molecular weight: | 39kDa |
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