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Western blot analysis of extracts of various cell lines, using PDHB antibody (A6943) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
PDHB Rabbit pAb
Catalog No：AB-A6943
Manufacturer：ABclonal
Synonyms：PDHB;PDHBD;PDHE1-B;PHE1B
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PDHB Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PDHB (NP_000916.2). |
| Gene ID: | 5162 |
| Swiss prot: | P11177 |
| Molecular weight: | 37kDa/39kDa |
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