Western blot analysis of extracts of various cell lines, using AP2A1 antibody (A6863) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
AP2A1 Rabbit pAb
Catalog No:AB-A6863
Manufacturer:ABclonal
Synonyms:AP2A1;ADTAA;AP2-ALPHA;CLAPA1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | AP2A1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human AP2A1 (NP_055018.2). |
Gene ID: | 160 |
Swiss prot: | O95782 |
Molecular weight: | 105kDa/107kDa |
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