Western blot analysis of extracts of various cell lines, using POLD2 antibody (A6834) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
POLD2 Rabbit pAb
Catalog No:AB-A6834
Manufacturer:ABclonal
Synonyms:POLD2
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | POLD2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3′ to 5′ exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-360 of human POLD2 (NP_006221.2). |
Gene ID: | 5425 |
Swiss prot: | P49005 |
Molecular weight: | 51kDa |
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