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Western blot analysis of extracts of HT-29 cells, using SPDEF antibody (A6747) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
SPDEF Rabbit pAb
Catalog No：AB-A6747
Manufacturer：ABclonal
Synonyms：SPDEF;PDEF;bA375E1.3
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | SPDEF Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human SPDEF (NP_036523.1). |
| Gene ID: | 25803 |
| Swiss prot: | O95238 |
| Molecular weight: | 35kDa/37kDa |
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