Western blot analysis of extracts of various cell lines, using RPS27 antibody (A6729) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
RPS27 Rabbit pAb
Catalog No:AB-A6729
Manufacturer:ABclonal
Synonyms:RPS27;MPS-1;MPS1;S27
Applications:WB,IHC
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RPS27 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-84 of human RPS27 (NP_001021.1). |
Gene ID: | 6232 |
Swiss prot: | P42677 |
Molecular weight: | 9kDa |
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