Western blot analysis of extracts of various cell lines, using POLG2 antibody (A6695) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
POLG2 Rabbit pAb
Catalog No:AB-A6695
Manufacturer:ABclonal
Synonyms:POLG2;HP55;MTPOLB;PEOA4;POLB;POLG-BETA;POLGB
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | POLG2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 216-485 of human POLG2 (NP_009146.2). |
Gene ID: | 11232 |
Swiss prot: | Q9UHN1 |
Molecular weight: | 54kDa |
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