Western blot analysis of extracts of various cell lines, using NSFL1C antibody (A6677) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
NSFL1C Rabbit pAb
Catalog No:AB-A6677
Manufacturer:ABclonal
Synonyms:NSFL1C;P47;UBX1;UBXD10;UBXN2C;dJ776F14.1
Applications:WB,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NSFL1C Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2). |
Gene ID: | 55968 |
Swiss prot: | Q9UNZ2 |
Molecular weight: | 28kDa/37kDa/40kDa |
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