Western blot analysis of extracts of various cell lines, using EIF5 antibody (A6583) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
EIF5 Rabbit pAb
Catalog No:AB-A6583
Manufacturer:ABclonal
Synonyms:EIF5;EIF-5;EIF-5A
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | EIF5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-235 of human EIF5 (NP_892116.2). |
Gene ID: | 1983 |
Swiss prot: | P55010 |
Molecular weight: | 49kDa |
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