Western blot analysis of extracts of BT-474 cells, using CD177 antibody (A6550) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
CD177 Rabbit pAb
Catalog No:AB-A6550
Manufacturer:ABclonal
Synonyms:CD177;HNA-2a;HNA2A;NB1;NB1 GP;PRV-1;PRV1;NB1GP
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CD177 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener’s granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human CD177 (NP_065139.2). |
Gene ID: | 57126 |
Swiss prot: | Q8N6Q3 |
Molecular weight: | 15kDa/27kDa/46kDa |
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