Western blot analysis of extracts of various cell lines, using Calpain small subunit 1 Antibody (A6539) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Calpain small subunit 1 Rabbit pAb
Catalog No:AB-A6539
Manufacturer:ABclonal
Synonyms:CAPNS1;CALPAIN4;CANP;CANPS;CAPN4;CDPS;CSS1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Calpain small subunit 1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 69-268 of human Calpain small subunit 1 (NP_001740.1). |
Gene ID: | 826 |
Swiss prot: | P04632 |
Molecular weight: | 28kDa |
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