Western blot analysis of extracts of various cell lines, using GLI2 antibody (A6510) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
GLI2 Rabbit pAb
Catalog No:AB-A6510
Manufacturer:ABclonal
Synonyms:GLI2;CJS;HPE9;PHS2;THP1;THP2;Gli2
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GLI2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. |
Immunogen: | Recombinant protein of mouse GLI2 |
Gene ID: | 14633 |
Swiss prot: | P10070 |
Molecular weight: | 168kDa |
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