Western blot analysis of extracts from normal (control) and DNMT3A knockout (KO) 293T cells, using DNMT3A antibody (A6503) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
DNMT3A Rabbit pAb
Catalog No:AB-A6503
Manufacturer:ABclonal
Synonyms:DNMT3A;DNMT3A2;M.HsaIIIA;TBRS;DNMT3A
Applications:WB,IF
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DNMT3A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human DNMT3A (NP_715640.2). |
Gene ID: | 1788 |
Swiss prot: | Q9Y6K1 |
Molecular weight: | 16kDa/81kDa/101kDa |
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