Western blot analysis of extracts of various cell lines, using POLE3 antibody (A6469) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
Western blot analysis of extracts from normal (control) and [KO Validated] POLE3 Rabbit pAb knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit pAb antibody (A6469) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] POLE3 Rabbit pAb
Catalog No:AB-A6469
Manufacturer:ABclonal
Synonyms:POLE3;CHARAC17;CHRAC17;YBL1;p17
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] POLE3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3). |
Gene ID: | 54107 |
Swiss prot: | Q9NRF9 |
Molecular weight: | 16kDa |
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