Western blot analysis of extracts of various cell lines, using POLR3K antibody (A6467) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
POLR3K Rabbit pAb
Catalog No:AB-A6467
Manufacturer:ABclonal
Synonyms:POLR3K;C11;C11-RNP3;My010;RPC10;RPC11;RPC12.5
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | POLR3K Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human POLR3K (NP_057394.3). |
Gene ID: | 51728 |
Swiss prot: | Q9Y2Y1 |
Molecular weight: | 12kDa |
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