Western blot analysis of extracts of various cell lines, using Coilin antibody (A6428) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Coilin Rabbit pAb
Catalog No:AB-A6428
Manufacturer:ABclonal
Synonyms:COIL;CLN80;p80-coilin;coilin;Coilin
Applications:WB,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Coilin Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 297-576 of human Coilin (NP_004636.1). |
Gene ID: | 8161 |
Swiss prot: | P38432 |
Molecular weight: | 62kDa |
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