Western blot analysis of extracts of various cell lines, using Pyruvate carboxylase (Pyruvate carboxylase (PC)) antibody (A6301) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Pyruvate carboxylase (PC) Rabbit pAb
Catalog No:AB-A6301
Manufacturer:ABclonal
Synonyms:PC;PCB
Applications:WB,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Pyruvate carboxylase (PC) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5′ UTRs, but encoding the same protein, have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human Pyruvate carboxylase (Pyruvate carboxylase (PC)) (NP_001035806.1). |
Gene ID: | 5091 |
Swiss prot: | P11498 |
Molecular weight: | 57kDa/129kDa |
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