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Western blot analysis of extracts of various cell lines, using DNTT antibody (A6254) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
DNTT Rabbit pAb
Catalog No：AB-A6254
Manufacturer：ABclonal
Synonyms：DNTT;TDT
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | DNTT Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3′-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-509 of human DNTT (NP_004079.3). |
| Gene ID: | 1791 |
| Swiss prot: | P04053 |
| Molecular weight: | 58kDa |
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