Western blot analysis of extracts of various cell lines, using SETDB1 antibody (A6145) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Western blot analysis of extracts of 293T cells, using SETDB1 antibody (A6145) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] SETDB1 Rabbit pAb
Catalog No:AB-A6145
Manufacturer:ABclonal
Synonyms:SETDB1;ESET;H3-K9-HMTase4;KG1T;KMT1E;TDRD21
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] SETDB1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-397 of human SETDB1 (NP_001230420.1). |
Gene ID: | 9869 |
Swiss prot: | Q15047 |
Molecular weight: | 44kDa/143kDa |
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