Western blot analysis of extracts of various cell lines, using Pumilio 1 antibody (A6108) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 15s.
Pumilio 1 Rabbit pAb
Catalog No:AB-A6108
Manufacturer:ABclonal
Synonyms:PUM1;HSPUM;PUMH;PUMH1;PUML1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Pumilio 1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3′ untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Pumilio 1 (NP_055491.1). |
Gene ID: | 9698 |
Swiss prot: | Q14671 |
Molecular weight: | 119kDa/124kDa/126kDa |
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