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Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (A6089) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 20s.
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Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (A6089) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human esophagus using HuR/ELAVL1 antibody (A6089) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using HuR/ELAVL1 antibody (A6089) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using HuR/ELAVL1 antibody (A6089) at dilution of 1:100 (40x lens).
[KO Validated] HuR/ELAVL1 Rabbit pAb
Catalog No：AB-A6089
Manufacturer：ABclonal
Synonyms：ELAV1;HUR;Hua;MelG;HuR / ELAVL1;ELAVL1
Applications：WB,IHC,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] HuR/ELAVL1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3′ untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (NP_001410.2). |
| Gene ID: | 1994 |
| Swiss prot: | Q15717 |
| Molecular weight: | 36kDa/38kDa |
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