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Western blot analysis of extracts of various cell lines, using EIF4G antibody (A6086) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60S.
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Immunohistochemistry of paraffin-embedded human breast cancer using EIF4G Rabbit pAb (A6086) at dilution of 1:100 (40x lens).
EIF4G Rabbit pAb
Catalog No：AB-A6086
Manufacturer：ABclonal
Synonyms：EIF4G1;EIF-4G1;EIF4F;EIF4G;EIF4GI;P220;PARK18
Applications：WB,IHC
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | EIF4G Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5′-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G (NP_886553.3). |
| Gene ID: | 1981 |
| Swiss prot: | Q04637 |
| Molecular weight: | 154kDa/158kDa/166kDa/171kDa/175kDa/176kDa |
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