Western blot analysis of extracts of various cell lines, using PABPN1 antibody (A6041) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
PABPN1 Rabbit pAb
Catalog No:AB-A6041
Manufacturer:ABclonal
Synonyms:PABPN1;OPMD;PAB2;PABII;PABP-2;PABP2
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PABPN1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3′ ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5′ end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PABPN1 (NP_004634.1). |
Gene ID: | 8106 |
Swiss prot: | Q86U42 |
Molecular weight: | 31kDa/32kDa/37kDa |
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