Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 1 (Carbonic Anhydrase 1 (CA1)) antibody (A1031) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Carbonic Anhydrase 1 (CA1) Rabbit pAb
Catalog No:AB-A1031
Manufacturer:ABclonal
Synonyms:CA1;CA-I;CAB;Car1;HEL-S-11
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Carbonic Anhydrase 1 (CA1) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human Carbonic Anhydrase 1 (Carbonic Anhydrase 1 (CA1)) (NP_001122303.1). |
Gene ID: | 759 |
Swiss prot: | P00915 |
Molecular weight: | 28kDa |
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