Western blot analysis of extracts of various cell lines, using PABPC4 antibody (A5948) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
PABPC4 Rabbit pAb
Catalog No:AB-A5948
Manufacturer:ABclonal
Synonyms:PABPC4;APP-1;APP1;PABP4;iPABP
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PABPC4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC4 (NP_003810.1). |
Gene ID: | 8761 |
Swiss prot: | Q13310 |
Molecular weight: | 69kDa/70kDa/72kDa |
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