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Western blot analysis of extracts of various cell lines, using NCL antibody (A5904) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human liver cancer using NCL Rabbit pAb (A5904) at dilution of 1:100 (40x lens).
NCL Rabbit pAb
Catalog No：AB-A5904
Manufacturer：ABclonal
Synonyms：NCL;C23;Nsr1;nucleolin
Applications：WB,IHC
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | NCL Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 461-710 of human NCL (NP_005372.2). |
| Gene ID: | 4691 |
| Swiss prot: | P19338 |
| Molecular weight: | 76kDa |
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