Western blot analysis of extracts of various cell lines, using MRPL28 antibody (A5897) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).
MRPL28 Rabbit pAb
Catalog No:AB-A5897
Manufacturer:ABclonal
Synonyms:MRPL28;MAAT1;p15
Applications:WB,IHC,IF,IP
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MRPL28 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MRPL28 (NP_006419.2). |
Gene ID: | 10573 |
Swiss prot: | Q13084 |
Molecular weight: | 30kDa |
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