Western blot analysis of extracts of various cell lines, using USP16 antibody (A5861) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
USP16 Rabbit pAb
Catalog No:AB-A5861
Manufacturer:ABclonal
Synonyms:USP16;UBP-M;UBPM
Applications:WB
Reactivity:Human,Mouse,Rat,Monkey
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | USP16 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP16 (NP_001001992.1). |
Gene ID: | 10600 |
Swiss prot: | Q9Y5T5 |
Molecular weight: | 46kDa/58kDa/91kDa/93kDa |
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