Western blot analysis of extracts of various cell lines, using SP100 antibody (A5851) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
SP100 Rabbit pAb
Catalog No:AB-A5851
Manufacturer:ABclonal
Synonyms:SP100;lysp100b
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SP100 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 181-480 of human SP100 (NP_001193631.1). |
Gene ID: | 6672 |
Swiss prot: | P23497 |
Molecular weight: | 50kDa/53kDa/78kDa/100kDa/101kDa |
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