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Western blot analysis of extracts of various cell lines, using SKIL antibody (A5844) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human mammary cancer using SKIL antibody (A5844) at dilution of 1:100 (40x lens).
SKIL Rabbit pAb
Catalog No：AB-A5844
Manufacturer：ABclonal
Synonyms：SKIL;SNO;SnoA;SnoI;SnoN
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | SKIL Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 420-684 of human SKIL (NP_001234937.1). |
| Gene ID: | 6498 |
| Swiss prot: | P12757 |
| Molecular weight: | 44kDa/46kDa/71kDa/74kDa/76kDa |
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