Western blot analysis of extracts of various cell lines, using PRKCQ Antibody (A5834) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PRKCQ Rabbit pAb
Catalog No:AB-A5834
Manufacturer:ABclonal
Synonyms:PRKCQ;PRKCT;nPKC-theta
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PRKCQ Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human PRKCQ (NP_006248.1). |
Gene ID: | 5588 |
Swiss prot: | Q04759 |
Molecular weight: | 67kDa/74kDa/81kDa |
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