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Western blot analysis of extracts of C6 cells, using HDAC8 antibody (A5829) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts from normal (control) and HDAC8 knockout (KO) HeLa cells, using HDAC8 antibody (A5829) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat testis using HDAC8 antibody (A5829) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spinal cord using HDAC8 antibody (A5829) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using HDAC8 antibody (A5829) at dilution of 1:100 (40x lens).
[KO Validated] HDAC8 Rabbit pAb
Catalog No：AB-A5829
Manufacturer：ABclonal
Synonyms：HDAC8;CDA07;CDLS5;HD8;HDACL1;MRXS6;RPD3;WTS
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] HDAC8 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1). |
| Gene ID: | 55869 |
| Swiss prot: | Q9BY41 |
| Molecular weight: | 15kDa/16kDa/17kDa/28kDa/29kDa/31kDa/41kDa |
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