Western blot analysis of extracts of various cell lines, using GOT1 antibody (A5822) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
GOT1 Rabbit pAb
Catalog No:AB-A5822
Manufacturer:ABclonal
Synonyms:GOT1;AST1;ASTQTL1;GIG18;cAspAT;cCAT
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GOT1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 244-413 of human GOT1 (NP_002070.1). |
Gene ID: | 2805 |
Swiss prot: | P17174 |
Molecular weight: | 44kDa/46kDa |
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