Western blot analysis of extracts of various cell lines, using ATG3 antibody (A5809) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
ATG3 Rabbit pAb
Catalog No:AB-A5809
Manufacturer:ABclonal
Synonyms:ATG3;APG3;APG3-LIKE;APG3L;PC3-96
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ATG3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human ATG3 (NP_071933.2). |
Gene ID: | 64422 |
Swiss prot: | Q9NT62 |
Molecular weight: | 35kDa |
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