Western blot analysis of extracts of HCT116 cells, using TOPBP1 antibody (A5781) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
TOPBP1 Rabbit pAb
Catalog No:AB-A5781
Manufacturer:ABclonal
Synonyms:TOPBP1;TOP2BP1
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | TOPBP1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2). |
Gene ID: | 11073 |
Swiss prot: | Q92547 |
Molecular weight: | 170kDa |
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