Western blot analysis of extracts of various cell lines, using DIAPH1 antibody (A5772) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
DIAPH1 Rabbit pAb
Catalog No:AB-A5772
Manufacturer:ABclonal
Synonyms:DIAPH1;DFNA1;DIA1;DRF1;LFHL1;SCBMS;hDIA1
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DIAPH1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1149-1263 of human DIAPH1 (NP_001073280.1). |
Gene ID: | 1729 |
Swiss prot: | O60610 |
Molecular weight: | 138kDa/140kDa/141kDa |
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