Western blot analysis of extracts of various cell lines, using Tryptophanyl-tRNA synthetase antibody (A5758) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Tryptophanyl-tRNA synthetase Rabbit pAb
Catalog No:AB-A5758
Manufacturer:ABclonal
Synonyms:WARS;GAMMA-2;IFI53;IFP53
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Tryptophanyl-tRNA synthetase Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Tryptophanyl-tRNA synthetase (NP_776049.1). |
Gene ID: | 7453 |
Swiss prot: | P23381 |
Molecular weight: | 48kDa/53kDa |
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